Because ettan dige system comprises many different products, references to the appropriate manuals for further details are made when necessary, see table 11. Ettan ipgphor 3 control software allows managing four ettan ipgphor 3 ief units simultaneously. Divided into compartments with a platform in the middle, the horizontal electrophoresis systems completely submerge plated gel platforms in a buffer. Features the ettan ipgphor 3 isoelectric focusing system includes. This system can run up to 12 ipg strips from 7cm to 24 cm. Ettan ipgphor ii isoelectric focusing system performs the first dimension of 2d gel electrophoresis, protein isoelectric focusing ief.
Ettan dige system user manual 18117317 summary proteins from prokaryotic or eukaryotic cells as discrete. This item includege ettan ipgphor 3 integrated isoelectric. Programmable functions include rehydration time, platform. To control multiple units from one computer, an optional junction box is necessary. Swivel the cams into the closed position under the external lip of the tray. For further information relating to sample preparation, please refer to the ettan dige user manual. The seconddimension electrophoresis separates the proteins on the basis of. A00603 ettan ipgphor ii isoelectric focusing system. Fully integrated equipment optimized to deliver high throughput, speed, reproducibility and high. Ettan ipgphor 3 ief system cytiva formerly ge healthcare life. Ge healthcare ettan daltsix user manual pdf download. Two glass plates are joined along one edge by a hinge strip of silicone rubber, and the vinyl side spacers 1. Pharmacia amersham ipgphor biotech ipgphor isoelectric focusing system. Connect only to coolant sources with regulated pressure.
Ettan ipgphor 3 is a fully integrated isoelectric focusing ief system optimized to deliver high throughput, speed, reproducibility and high proteinloading capacity. The ettan ipgphor 3 control panel allows up to 10 user defined ief protocols, each with nine steps. Used ge healthcare ettan ipgphor3 system chemistry. This system is pulled out from the working environment. The user can create, save, and edit protocols and also pause during a run to edit a protocol in real time. If working with a sample in an unknown buffer, the sample should be precipitated and resuspended in an ettan dige systemcompatible buffer. The product must also be used in the same state as it was delivered from ge healthcare and connected only to other ce labeled ge healthcare modules. Ettan ipgphor 3 isoelectric focusing system, ge healthcare. Ipgphor manifold light 11002688 routine applications. Read 2d electrophoresis principles and methods, ettan. To view your gsa or va contract pricing, log in using your account number, or become a. The product fulfills valid directives and standards when used within the conditions specified in the user manual. Ettan ipgphor 3 control software user manual 11003459 ettan ipgphor 3 instrument user manual 11003458 ettan ipgphor cup loading manifold user manual 11003460 2d electrophoresis principles and methods 80642960 ettan dalttwelve system user manual 80647653.
Ettan ipgphor 3 isoelectric focusing unit 45002623 unit to run first. If you are viewing this page as a nonregistered user, the prices displayed is list price. Match together the two spot maps using the decyder 2d software and create a pick list for all the spots corresponding to the cy 5 labeled cell surface proteins. For creating, editing, and running protocol programs from the ettan ipgphor control panel, please refer to the ettan ipgphor 3 instrument user manual. The ettan ipgphor ii from ge healthcare is the ideal option for obtaining perfectly resolved first dimensions of 2d gels. Ettan ipgphor 3 control software can be used to control up to four ettan ipgphor 3 units at one time, each running a different program. Ge healthcare ettan ipgphor 3 beta gamma counter for sale. The uninstall ettan ipgphor ii control software program will start.
For detailed instructions on installation and usage of this software, refer to the ettan ipgphor 3 control software user manual. Ge healthcare manifold kit for ettan ipgphor 3 isoelectric focusing unit. The protein facility provides equipment and expertise for the analysis, characterization and synthesis of proteins and peptides and is open to both internal and external investigators. Imaging twodimensional protein gels stained with sypro ruby. The product must also be used in the same state as it was delivered from ge healthcare and connected only to other ce labeled ge healthcare modules or other products as recommended. Equilibrate the focused ipg strips in sds equilibration. Ettan ipgphor 3 ief system cytiva formerly ge healthcare. Data file 28404198 aa ettan ipgphor 3 ettan ipgphor 3. The manufacturer claims that the system provides reproducibility. Equilibrate the focused ipg strips in sds equilibration buffer plus 1. Builtin purification knowledge combines software, hardware, and prepacked columns to eliminate timeconsuming manual tasks. Ettan ipgphor 3 ief system is a fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2d electrophoresis run up to 12 ipg strips 7, 11, 18, or 24 cm simultaneously using ettan ipgphor manifold, ettan ipgphor manifold light, or.
The manufacturer claims that the system provides reproducibility, reliability, speed, accuracy, high protein loading capacity, and high throughput, and in my experience, the system really stands up to their claim. Carver department of biochemistry, biophysics and molecular biology and the plant. The software can be used to control up to four ettan ipgphor 3 ief units. Protein facility office of biotechnology iowa state. Ettan ipgphor 3 operating instructions 28963551 ac 5 operation 5. Ge ettan ipgphor 3 integrated isoelectric focusing unit. Ettan ipgphor 3 control software fig 22, with an external personal computer windows connected via a serial port, can be used to control up to four ettan ipgphor 3 units simultaneously, each running a different set of run parameters. The ettan dalt ii system is designed to handle multiple large seconddimension gels in a simple, ef. For use with equipment ettan ipgphor 3 isoelectric focusing unit. Ettan ipgphor 3 isoelectric focusing system appendix i, extracted from 2d electrophoresis, ge healthcare, 2010 table 20 lists possible problems that might be encountered during ief and how to solve them, and table 21. Ge healthcare ettan ipgphor3 system used verified powerup. Ettan ipgphor 3 control software ettan ipgphor 3 control software generates professional graphical reports with a large user interface for programming and editing. Inserting gels into the ettan dalt electrophoresis buffer tank.
Page 1 ge healthcare ettan daltsix electrophoresis system user manual page 3 12 psig. Ettan ipgphor 3 isoelectric focusing unit cytiva formerly. Ettan ipgphor 3 ief system is a fully integrated isoelectric focusing system optimized to deliver speed and reliability in the first dimension of 2d electrophoresis. The default installation of the ettan ipgphor ii control software will be on the local c drive within the folder titled amersham biosciences. Ipg gel strips using the ipgphor or multiphor ii ief systems. Ettan ipgphor 3 control software allows easy programming and. Data file 28404198 aa ettan ipgphor 3 ettan ipgphor 3 isoelectric focusing system the ettan ipgphor 3 is a fully integrated isoelectric focusing ief system optimized to deliver high throughput.
Amersham pharmacia biotech 3 ipgphor isoelectric focusing system user manual control panel the ipgphor is controlled by a 7key membrane keypad. For detailed instructions on installation and usage of ettan ipgphor control software, and also on computer instrument connection, please refer to the ettan ipgphor software user manual. Read the instruction manual fully before starting up the um3b unit. The ettan ipgphor cup loading manifold user manual contains instructions for firstdimension isoelectric focusing of proteins on ipg strips. Ge healthcare ettan ipgphor 3 isoelectric focusing system ipgphor 3 isoelectric focusing system ief electrophoresis systems electrophoresis equipment. The manual also contains complete instructions for running the instrument, including standard protocols for beginners. Ettan ipgphor 3 control software allows easy programming and viewing of run data. Please read this entire manual to fully understand the safe and effective use of this product. Used beta gamma counter ge healthcare ettan ipgphor 3. Ettan ipgphor ii from ge healthcare biocompare product. Ettan ipgphor user manual 11003460 ad 19 3 operation 3.
Ettan ipgphor 3 with ettan ipgphor manifold offers a. The protein facility is supported by the office of biotechnology, the roy j. Ettan ipgphor 3 isoelectric focusing unit 1 11003364 ipgphor manifold ceramic tray 1 80649857. Data file 28937442 aa 2d electrophoresis ipgbox and ipgbox kit. Ettan ipgphor 3 control software user manual 11003459. In each compartment, electrodes produce the electric field to cause. Increased safety features ensure safe highvoltage runs, and with total startstop control from your pc, ief runs have never been easier or more reproducible. Pick cell surface proteins using the ettan spot handling station, extract from the gel plugs, and trypsinate using the ettan digester. Ettan daltsixelectrophoresis system function and description in 2d electrophoresis, proteins are separated according to isoelectric point by isoelectric focusing, most reliably on immobiline drystrip immobilized ph gradient ipg gel strips using the ipgphor or multiphor ii ief systems. Sample preparation for ettan dige system applications sigma. Please read this entire manual to fully understand the safe. Synchronized solutions for better 2d electrophoresis. Data file 28937442 aa 2d electrophoresis ipgbox and.
Ettan ipgphor user manual 11003460 ad 3 operation 3. Ge healthcare manifold kit for ettan ipgphor 3 isoelectric. Data file 28937442 aa 2d electrophoresis ipgbox and ipgbox kit ipgbox insert. Ettan ipgphor 3 isoelectric focusing system function and description 1 ettan ipgphor 3 instrument user manual 11003458 edition ab control panel the ettan ipgphor 3 is controlled by a 7key membrane keypad. The ettan daltsixsystem uses the same cassettes as the ettan dalttwelve electrophoresis system see fig 3.
Selective labelling of cellsurface proteins using cydye. Ettan ipgphor ii isoelectric focusing system amersham. Ettan ipgphor isoelectric focusing system ipgphor function and description um 80641535 p2 control panel negative cathode electrode area positive anode electrode area cover pressure block sample application wells base 1. Ge healthcare manifold kit for ettan ipgphor 3 isoelectric focusing unit complete manifold kit. Ettan ipgphor3 ief system 7cm strip protocols youtube. The software can be used to control up to four ettan ipgphor 3 ief units simultaneously, each running a. Ettan dige system users manual university of alberta.
Ettan ipgphor 3 manifold the manifold is designed to handle ief and subsequent equilibration for up to 12 ipg strips. Ipgphor 3 instrument user manual and ettan ipgphor 3 safety handbook prior to operation of the ettan. Its objective is to provide everything needed for successful operation. Ettan ipgphor ii isoelectric focusing system amersham biosciences version3. Ettan ipgphor 3 isoelectric focusing unit 11003364 unit to run first dimension ief. Ief using ettan ipgphor 3 isoelectric focusing system and. Data file 28937442 aa 2d electrophoresis ipgbox and ipgbox kit ipgbox. Ettan ipgphor 3 isoelectric focusing unit 11003364 unit to run first dimension ief ipgphor manifold, complete 80649838 for the most stringent applications. Ge healthcare ettan ipgphor 3 isoelectric focusing system.
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